Abstract
Background: Nowadays, the use of herbal medicines is very substantial in the treatment of cancer. One of the most common cancers among
women is Cervical cancer. Due to the side effects of some chemical drugs, the use of medicinal plants with minimum side effects and
interference has been considered. Rosemary (R. Official L.), a member of the Lamiaceae family, is known for its antioxidant properties. One
of the main compounds for antioxidant activity is Carnosic acid (CA), a polyphenol with antitumor biological activities.
Method: This study investigated the effect of Carnosic acid at different concentrations (2.5, 5, 10, 15, 20, 25, and 50) mg/ml on a Cervical
cancer cell line (Hela). Cytotoxicity and growth inhibitory power (IC50) were calculated using the MTT test, Prism software. The real-time
PCR method investigated TGF-β1 gene expression on Hela cancer cell lines and cells treated with Carnosic acid. To assess and quantify
oxidative stress and apoptotic behavior, we utilized a plate reader and the appropriate kit to measure catalase activity (CAT) level by detecting
570 nm light absorption.
Results: Cytotoxicity assay (MTT) showed the antitumor activity of the group treated with CA compared to the control group (Hela cells
without treatment). Gene expression analysis based on RT-PCR showed downregulation of the TGF-β gene and catalase activity reduction
in cells treated with Carnosic acid compared to untreated cells.
Conclusion: Carnosic acid has been introduced as a promising formula for treating cervical cancer.
Main Subjects
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