Print ISSN: 0253-0759

Online ISSN: 2413-4414

Keywords : Gastric Ulcer


THE INTERACTION BETWEEN NIGELLA SATIVA FIXED OIL AND RANITIDINE ON

Jawad H. Ahmed; Ahmed H. Naema; Nabeel A. Ali

The Medical Journal of Basrah University, Volume 30, Issue 1, Pages 19-25
DOI: 10.33762/mjbu.2012.64010

Background: The use of herbal preparations has increased dramatically, making drug interactions with these
preparations a major health concern, especially as herbal medications are usually not subjected to the same
regulations as prescription drugs
Aim: as a potential drug-herb interaction is possible, this study was designed to investigate the interaction between
Nigella sativa (NS) and ranitidine (R) on absolute ethanol induced gastric mucosal damage in rabbit
Materials and Methods: Five groups of rabbits (6 each) were used. Acute gastric ulcerations were induced by ethanol
through a stomach tube. The oil of NS was given orally, ranitidine by (IM), combination of NS+R or normal saline
were given 1 hour before ethanol. Ulcer index, serum and stomach tissue MDA, gastric volume and pH, and
histopathology were evaluated.
Results: Monotherapy of NSoil or R reduced the mean ulcer index from 91.7±19.4mm in the control group to
43.3±8.7 and 22.5±9.4mm for NS and R treatment respectively. There were significant reductions in serum and
stomach tissue MDA and in gastric secretion. When NS and R were given in combination the anti-ulcer effect of both
disappeared. This was associated with increased MDA levels in stomach tissue, but not serum. The pH of stomach
content was also changed toward ethanol treated values.
Conclusion: These findings document the gastro-protective potential of NS against ethanol-induced gastric ulcer.
There was a significant NS-R interaction manifested as failure of the combination to inhibit ulcers formation. Until
further wider studies are available to confirm such interaction, the simultaneous use of Nigella sativa and ranitidine
should be discouraged.

EVALUATION OF THE GASTROPROTECTIVE EFFECT OF MISOPROSTOL, CHITOSAN AND THEIR COMBINATION ON INDOMETHACIN INDUCED GASTRIC ULCER IN RATS.

Jawad H. Ahmed; Jala; a A. Salman Al-Ahmed; Eman A. Al-Masoodi

The Medical Journal of Basrah University, Volume 29, Issue 1, Pages 1-8
DOI: 10.33762/mjbu.2011.49471

ABSTRACT
Background: The study was designed to evaluate the anti-ulcer effect of chitosan, misoprostol, and their combination on gastric ulceration induced by indometacin in rats.
Methods and experimental design: Chitosan was prepared from shrimp shells waste products. Thirty rats were divided into 5 groups, 6 rats each. Rats in group 1 (control) were given solid sugar and distilled water for 3 days; group 2 were treated by indometacin (25mg/rat) ; group 3,4, and 5 were treated by misoprostol, chitosan, and by the combination chitosan and misoprostol respectively before treatment with indometacin. Blood were collected before sacrificing the animals and used for estimation of MDA, a marker of oxidative stress. The stomachs were prepared for estimating the total gastric area, ulcerated area, tissue MDA, mucin production as well as for histopathological examination.
Results: Indometacin produced gastric ulcers, and increased the total gastric area in all animals. These effects were associated with a significant elevation of MDA levels in the blood and in stomach tissues, and a significant reduction in mucin production. Misoprostol, chitosan and their combination protected gastric mucosa since they significantly reduced ulcer index. Moreover, the observed anti-ulcer effect was more with the combination in comparison to monotherapy of misoprostol or chitosan.
Treatments by misoprostol, chitosan and their combination before indometacin significantly reduced blood and tissue MDA levels and increased mucin production.
Conclusion: Chitosan, misoprostol and their combination have gastroprotective effects against indometacin-induced gastric ulceration in rats.