Keywords : Nigella sativa

The anti ulcer effect of omeprazole is modified by Nigella sativa (Black Cumin) in ethanol induced gastric ulceration in rabbits

Jawad Hassan Ahmed; Ali Mohammed Al-Rawaq

The Medical Journal of Basrah University, 2020, Volume 38, Issue 2, Pages 85-98
DOI: 10.33762/mjbu.2020.127147.1018

Background: An interaction has been reported between Nigella Sativa (NS) and ranitidine (RAN) on gastric ulceration induced by ethanol in rabbits; the combination NS and RAN caused disappearance of anti-ulcer effect of NS or RAN.
Objective: to investigate interaction of NS with a proton pump inhibitor omeprazole (OMP) on ethanol induced gastric ulceration in rabbits.
Methods: 24 mature rabbits were divided into 4 groups. The animals were fasted for 48 hours then treated as follow: group 1, 2, 3 and 4 were treated respectively with normal saline (oral), NS oil (10ml/kg) orally, OMP (20mg/kg) IP, and NS+ OMP. One hour later, animals were given absolute ethanol orally; and sacrificed 3 hours later for estimation of Ulcer index (UI), gastric pH, malondialdehyde (MDA), glutathione (GSH), histamine (HIS) levels in serum and gastric tissue.
Results: Ethanol induced gastric ulceration in all animals with an UI of 10 ± 0.11 mm2. This effect was paralleled with reduction in gastric pH, increased MDA and HIS and reduction in GSH. UI was reduced to 5.13 ± 0.68 mm2 in NS group, P value = 0.07 and to around zero in OMP group. NS or OMP treatment resulted in reduction in serum and tissue MDA and HIS levels and increased in GSH and gastric pH levels. In NS + OMP treated group, UI became higher than OMP group with MDA and HIS tended to rise and GSH and gastric pH declined.
Conclusion: NS + OMP diminished the gastro-protective effect of either NS or OMP.


Jawad H. Ahmed; Ahmed H. Naema; Nabeel A. Ali

The Medical Journal of Basrah University, 2012, Volume 30, Issue 1, Pages 19-25
DOI: 10.33762/mjbu.2012.64010

Background: The use of herbal preparations has increased dramatically, making drug interactions with these
preparations a major health concern, especially as herbal medications are usually not subjected to the same
regulations as prescription drugs
Aim: as a potential drug-herb interaction is possible, this study was designed to investigate the interaction between
Nigella sativa (NS) and ranitidine (R) on absolute ethanol induced gastric mucosal damage in rabbit
Materials and Methods: Five groups of rabbits (6 each) were used. Acute gastric ulcerations were induced by ethanol
through a stomach tube. The oil of NS was given orally, ranitidine by (IM), combination of NS+R or normal saline
were given 1 hour before ethanol. Ulcer index, serum and stomach tissue MDA, gastric volume and pH, and
histopathology were evaluated.
Results: Monotherapy of NSoil or R reduced the mean ulcer index from 91.7±19.4mm in the control group to
43.3±8.7 and 22.5±9.4mm for NS and R treatment respectively. There were significant reductions in serum and
stomach tissue MDA and in gastric secretion. When NS and R were given in combination the anti-ulcer effect of both
disappeared. This was associated with increased MDA levels in stomach tissue, but not serum. The pH of stomach
content was also changed toward ethanol treated values.
Conclusion: These findings document the gastro-protective potential of NS against ethanol-induced gastric ulcer.
There was a significant NS-R interaction manifested as failure of the combination to inhibit ulcers formation. Until
further wider studies are available to confirm such interaction, the simultaneous use of Nigella sativa and ranitidine
should be discouraged.