731236,,


Association between Taq1 rs: 731236 SNP of VDR gene and risk factor among Sudanese patients with breast cancer

Amira S. Khalafalla; Abuagla Dafalla; Yousif Abdelhameed Mohammed; Adam D. Abakar; Mergani Adil; GadAllah osman Modawe

The Medical Journal of Basrah University, 2021, Volume 39, Issue 2, Pages 104-110
DOI: 10.33762/mjbu.2021.130830.1082

Abstract:
Background: Breast cancer is the most frequent cancer in women globally as well asSudan, it has risen to become the second commonest cause of death in women.Vitamin D receptor (VDR) is a ligand-dependent transcription factor. Vitamin D-ligandedVDR, has anti-proliferative properties in a variety of tumor types by inducing cell cycle arrest, senescence, differentiation, and death. The role of VDR TaqI polymorphism is currently unknown. However, studies suggest that these polymorphisms may affect messenger RNA (mRNA) stability.
Methods and patients: This case control 198 participants, (97) breast cancer patients and another 101control group. Data was collectedby a questionnaire. 2 ml of venous blood was collected and stored at -20 till DNA extraction. Phenol chloroform method was used for DNA extraction. VDR TaqI was examined and genotyped using CTPP-PCR after designing of suitable primers and PCR condition. Then data was analyzed statistically using the SPSS program (version 21) and the SNPS tats online tool.
Results: The three genotypes reported in this study for TaqI SNP (CC, TC, and TT) were evenly distributed throughout cases and controls, so according to this findings there is no statistically significant association of this SNP with breast cancer risk (p. value 0.650) (OR(95%CI) 1.39 (0.64-3.00), 1.00 (0.54-1.87), 1.00).
Conclusions: This study found no association of TaqI polymorphism and breast cancer risk factors