Keywords : vitamin D

Serum vitamin D level, measured by two methods, in a sample of normal subjects in Basrah

Huda A Yaqoob; Nazar S Haddad; Abdullah M Jawad

The Medical Journal of Basrah University, 2019, Volume 37, Issue 2, Pages 106-114
DOI: 10.33762/mjbu.2019.163361

Background: Low vitamin D levels had been reported to be common in normal subjects worldwide. Studies in the Middle East had reported extremely low levels of serum vitamin D, despite high exposure to sunlight. Aim: To estimate vitamin D serum concentration in a sample of apparently healthy subjects from Basrah, by 2 methods (chemiluminescent and fluorescent assays). Methods: The study was carried out on apparently healthy subjects during the period from September 2018 to February 2019. Quantitative determination of the total 25-hydroxy vitamin D in serum was made using chemiluminescent microparticle immunoassay and enzyme-linked fluorescent assay. Other parameters (calcium, phosphorus, alkaline phosphatase, parathyroid hormone, hemoglobin, and erythrocyte sedimentation rate and body mass index) were also measured. Results: The mean level of vitamin D measured by the two methods, was 11.57±6.63 ng/ml and 13.31±6.52 ng/ml by chemiluminescent and fluorescent assays respectively. The prevalence of vitamin D deficiency was high, where more than 80% of the 57 subjects had vitamin D level below 20 ng/ml. If the cut-off point of vitamin D deficiency was taken as 10 ng/ml, around 46% of the subjects were found deficient in both methods. Although the two methods of vitamin D assay were well correlated with each other, fluorescent assay gave, on average, a significantly higher levels compared with the chemiluminescent method. Serum parathyroid hormone, showed a negative correlation with vitamin D serum levels. After excluding children and females, no significant difference was found between adult smokers and non-smokers when vitamin D was measured by both methods.Conclusion: Vitamin D deficiency is common (> 80%) among normal subjects. The enzyme-linked fluorescent assay resulted in higher mean level than chemiluminescent assay. The use of a deficiency cut-off point of 10 ng/ml may be more appropriate.

Assessment of vitamins D and B12 after laparoscopic sleeve gastrectomy

Rula Abdual-Hafed; Issam Merdan; Abdul Kareem Alebadi

The Medical Journal of Basrah University, 2019, Volume 37, Issue 2, Pages 68-73
DOI: 10.33762/mjbu.2019.163356

Background: Obesity is a big health problem due to its related diseases, so laparoscopic sleeve gastrectomy is one of the bariatric surgeries which have a role in the weight reduction, but it affects the micronutrients concentrations including vitamins D and B12.Aims: To assess concentrations of vitamins D and B12 prior to laparoscopic sleeve gastrectomy and post-surgery in first, third, sixth and twelve months after the surgery by taking blood samples in this scheduled follow up visits.Patients and Methods: This study was carried out in department of surgery at Al-Sadr Teaching Hospital in Basrah, Iraq from June 2016 to June 2017.It include 82 patients from both gender and from different age groups. Detection of Vitamins D and B12 deficiency pre and postoperative periods, in every follow up visits were by taking a samples of blood and statistical calculation of vitamins values.Results: There were no significant differences related to the age groups but there were a significant reduction of both vitamins in relation to sex and time (pre and postoperatively). The deficiency of vitamin D before surgery mean (35.18) which decreased then increased reaching (37.46) in the end of first year post surgery which was a significant P value 0.045, while the deficiency of vitamin B12 prior to surgery which was 51.90 then after surgery decreased then increased reaching 51.58 by the end of first year post surgery which was non-significant P-value 0.490 (P value < 0.05 significant).Conclusions: This study determined the presence of deficiencies of both vitamins D and B12 before and after laparoscopic sleeve gastrectomy during first year post surgery, so it is recommended for giving vitamin D prior to surgery, while vitamin B12 was not given.