Print ISSN: 0253-0759

Online ISSN: 2413-4414

Keywords : Chitosan


EVALUATION OF THE GASTROPROTECTIVE EFFECT OF MISOPROSTOL, CHITOSAN AND THEIR COMBINATION ON INDOMETHACIN INDUCED GASTRIC ULCER IN RATS.

Jawad H. Ahmed; Jala; a A. Salman Al-Ahmed; Eman A. Al-Masoodi

The Medical Journal of Basrah University, Volume 29, Issue 1, Pages 1-8
DOI: 10.33762/mjbu.2011.49471

ABSTRACT
Background: The study was designed to evaluate the anti-ulcer effect of chitosan, misoprostol, and their combination on gastric ulceration induced by indometacin in rats.
Methods and experimental design: Chitosan was prepared from shrimp shells waste products. Thirty rats were divided into 5 groups, 6 rats each. Rats in group 1 (control) were given solid sugar and distilled water for 3 days; group 2 were treated by indometacin (25mg/rat) ; group 3,4, and 5 were treated by misoprostol, chitosan, and by the combination chitosan and misoprostol respectively before treatment with indometacin. Blood were collected before sacrificing the animals and used for estimation of MDA, a marker of oxidative stress. The stomachs were prepared for estimating the total gastric area, ulcerated area, tissue MDA, mucin production as well as for histopathological examination.
Results: Indometacin produced gastric ulcers, and increased the total gastric area in all animals. These effects were associated with a significant elevation of MDA levels in the blood and in stomach tissues, and a significant reduction in mucin production. Misoprostol, chitosan and their combination protected gastric mucosa since they significantly reduced ulcer index. Moreover, the observed anti-ulcer effect was more with the combination in comparison to monotherapy of misoprostol or chitosan.
Treatments by misoprostol, chitosan and their combination before indometacin significantly reduced blood and tissue MDA levels and increased mucin production.
Conclusion: Chitosan, misoprostol and their combination have gastroprotective effects against indometacin-induced gastric ulceration in rats.

LOCALLY MADE CHITOSAN COVALENTLY LINKED TO INDOMETHACIN: A NOVEL APPROACH TO FORMULATE SUSTAINED RELEASE INDOMETHACIN CAPSULE IN BASRAH

Akhlas F. Thajeel; Jawad H. Ahmed

The Medical Journal of Basrah University, Volume 26, Issue 1, Pages 6-14
DOI: 10.33762/mjbu.2008.48324

ABSTRACT
The sustained release capsules are pharmaceutical dosage form used for the treatment of chronic rather than acute
diseases and are useful because of its convenience and less side effects. This work describes an attempt to formulate
indomethacin sustained release capsule using indomethacin covalently linked to chitosan. Chitosan was prepared by
alkaline -N-deacetylation of chitin, which was isolated from the shells of local shrimps. The yield of chitosan was
found to be 80%. This polymer was found to be white crystal, odorless and the IR spectroscopy of it was similar to a
standard reference. Chitosan has been covalently linked to indomethacin and the ratio of drug to polymer was found
to be 5:1. The chemical interaction between the drug and the polymer was proved by IR studies. In vitro release at
different pH were performed and the release of indomethacin in the prepared sustained release capsule were tested
and compared with conventional indomethacin capsule INDOMIN-25mg, and a commercial sustained release capsule
INDO-75-SR. The release of indomethacin in our product was completed within 12 hours, while the conventional
INDOMIN capsule and commercial INDO-75-SR capsule released within 2.5 and 5 hours respectively. In conclusion a
cheap, locally made chitosan can be used in formulation of sustained release capsules by covalently linked to drugs
containing carboxyl group (like indomethacin) or can be modified to have a carboxyl group.